Resveratrol Attenuates Endoplasmic Reticulum Stress-Induced Cell Death and Results in Functional Improvement after Traumatic Brain Injury in Mice

Volume 7, Issue 2, April 2022     |     PP. 41-55      |     PDF (2120 K)    |     Pub. Date: March 24, 2022
DOI: 10.54647/biology18214    80 Downloads     5780 Views  

Author(s)

Mark Nyanzu, Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China ; Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China ; St Georges university hospital, London Uk
Zhezhe Sun, Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China ; Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
Felix Siaw-Debrah, Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China ; Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
Ying Zhang, Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
Xiaohong Zhu, Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China ; Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
Su Yang, Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
Haoqi Ni, Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China ; Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
Xiao Lin, Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China ; Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
Qichuan ZhuGe, Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China ; Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
Lijie Huang, Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China ; Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China

Abstract
Resveratrol (RSV), a polyphenol antioxidant, has been reported to function as a neuroprotector. We explored the molecular mechanisms that underlines the anti-inflammatory activity of RSV in traumatic brain injury (TBI) in mice relevant to endoplasmic reticulum stress (ERS). By establishing three experimental groups (sham, TBI, and TBI+RSV), we examined the effects of RSV after TBI on ERS and caspase-12 apoptotic pathways cascades. The protein expressions levels of C/EBP homologous protein (CHOP), glucose regulated protein 78kD (GRP78), caspase-3, and caspase-12 in cortical brain tissues were evaluated by western blotting. The cortical brain tissues were also subjected to a qPCR analysis of mRNA expression of tumor necrosis factor (TNF)-α and interleukin (IL)-1β. Additionally, immunofluorescence staining was used to determine the expression of GRP78 in microglia (ionized calcium binding adaptor molecule 1;Iba-1) and neurons (neuronal nuclei;NeuN). The neurological function of the mice was analyzed based on modified neurological severity scores (mNSS). After RSV treatment, the expression of CHOP, GRP78, caspase-3 and caspase-12 decreased, and qPCR results showed downregulation of TNF-α and IL-1β. Immunofluorescence stain demonstrated Iba-1+/GRP78+ and NeuN+/GRP78+ cells decreased after RSV treatment. The mNSS analysis indicated improvement following RSV treatment. RSV alleviated neuro-apoptosis by inhibiting the ERS signaling pathway and also improved the neurological function of mice with TBI.

Keywords
Traumatic brain injury; Resveratrol; Endoplasmic reticulum stress; Apoptosis; Neuroinflammation

Cite this paper
Mark Nyanzu, Zhezhe Sun, Felix Siaw-Debrah, Ying Zhang, Xiaohong Zhu, Su Yang, Haoqi Ni, Xiao Lin, Qichuan ZhuGe, Lijie Huang, Resveratrol Attenuates Endoplasmic Reticulum Stress-Induced Cell Death and Results in Functional Improvement after Traumatic Brain Injury in Mice , SCIREA Journal of Biology. Volume 7, Issue 2, April 2022 | PP. 41-55. 10.54647/biology18214

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